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Mikroba sangat dipengruhi oleh kondisi lingkungannya, baik lingkungan biotik maupun abiotik. Jadi biofertilizer yang cocok di daerah sub tropis belum tentu efektif di daerah tropis. Demikian juga biofertilizer yang efektif di Indonesia bagian barat, belum tentu juga efektif di wilayah Indonesia bagian timur.Berdasarkan studi dari Universitas Califo